考察茵栀黄复方中抗胆汁淤积的主要药效成分，从胆汁酸代谢/炎症通路角度探讨其抗胆汁淤积性肝损伤作用的新机制。选用雄性健康129S/V小鼠，分成正常对照组、ANIT诱导模型对照组、绿原酸组、栀子苷组、黄芩苷组、滨蒿内酯组，采用液相色谱-质谱多反应监测技术开展茵栀黄复方注射液多组分血浆药动学轮廓分析和系统暴露测量；同时检测肝功能、肝组织病理学变化、胆汁酸代谢相关基因和炎症相关基因的mRNA表达及炎症相关
A rapid and simple background-free high-performance liquid chromatography/ electrospray ionization tandem mass spectrometry (HPLC/ESI-MS/MS) approach has been developed for simultaneously determining
采用放射性标记的方法：研究生物大分子多糖MP-1关节腔内药动学.以14C标记葡萄糖与普通葡萄糖作为混合碳源,通过发酵对MP-1进行放射性标记.给予大鼠关节腔注射0.25、0.5、1 mg/只3个剂量的14C标记MP-1,测定给药后不同时间关节腔内放射性残留.结果：显示通过发酵可成功制备比活度为7.27 μ Ci/mg,放化纯度为99.5％的放射性标记MP-1样品.关节腔注射MP-1 1 mg/只,
目的：对枸橼酸莫沙必利注射液进行临床前药代动力学评价.方法：：建立液相色谱-串联质谱(LC-MS/MS)法同时测定比格犬/大鼠血浆及组织中莫沙必利及两种代谢产物，并考察静脉注射给药相对于口服对莫沙必利体内过程的影响.样品经乙酸乙酯萃取后，以Eclipse Plus C18色谱柱分离，以甲醇-0.5％冰乙酸溶液为流动相.采用电喷雾离子源，多重反应监测模式对目标物离子监测.
Tolvaptan is an orally active vasopressin V2 receptor antagonist clinically used in the treatment of hyponatremia.Recently, U.S.FDA issued a black box warning for tolvaptan due to its high risk of liv
The db/db mice were a strain of mutant line from C57BL/6J mice, and it is always used as a human type 2 diabetes mellitus animal model.
Many plant-derived glycosides are used as medications.It is common that these glycosides show poor intestinal absorption but their metabolites generated by intestinal microflora demonstrate strong bio
Paeoniflorin (PF) and albiflorin (AF) are the main components of total peony glucosides (TPG), which has been widely used as the valuable monoterpene glycosides in Paeonia lactiflora Pall and have man
Objective Boswellia serrata was widely used in traditional medicine as an anti-inflammatory agent, 11-keto-β-boswellic acid (KBA) and 3-acetyl-11-keto-β-boswellic acid (AKBA) were the most potent BAs.
目的 确定人肝脏微粒体中蟾毒它灵的主要氧化代谢途径,并对参与其代谢的细胞色素P450酶亚型及其机制进行研究.方法 采用肝微粒体、重组酶体外代谢的孵育方法和液相色谱-串联质谱(LC-MS/MS)技术,分析孵育体系中蟾毒它灵及其代谢物,并使用特异性抑制剂、酶反应动力学和相关性分析实验进行验证实验,初步判断蟾毒它灵的主要代谢途径及其涉及的CYP同工酶,并用分子对接方法验证其代谢机理.结果 蟾毒它灵在人肝