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Microelectrode array (MEA),because of its advantages such as high sensitivity and signal-to-noise ratio (vs.macroelectrode),[1] has been given significant attentions in the fields of biological analysis,environmental monitoring and disease diagnosis.[2] Graphene has demonstrated its potential for next generation electronic device due to its fascinating physicochemical properties including extremely high carrier mobility,high electro-mechanical modulation and chemical activity.In this study,we develop a new technique to fabricate a reduced graphene oxide (rGO)-based MEA with scale-up and low-cost microfabrication technology (Fig.1).