U2AF1 mutations in Chinese patients with acute myeloid leukemia and myelodysplastic syndrome

• 作 者: QianJun;MaJi-chun;ChenXing-xing;YaoDong-ming;LinJiang;QianWei;WangCui-zhu;ChaiHai-yan;YangJing;LiYun;DengZhao-qun;
• 机 构: Affiliated People's Hospital of Jiangsu University
• 出 处: 肿瘤多学科综合诊治新进展学术研讨会
• 发表日期: 2012年期

　　Objective Somatic mutations of U2AF1 gene have recently been identified in myelodysplasfic syndrome (MDS) and acute myeloid leukemia (AML).In this study,we analyzed the frequency and clinical impact of U2AF1 mutations in a cohort of 452 Chinese patients with myeloid neoplasms.Methods PCR and high-resolution melting analysis (HRMA) were performed to detect the hotspot mutations of U2AF1 gene (Ser34 and Q157).Results Mutations in U2AF1 were found in 2.5% (7/275) of AML and 6.3% (6/96) of MDS patients,but in none of 81 CML.All mutations were heterozygous missense mutations affecting codon S34 or Q157.There was no significant association of U2AF1 mutation with blood parameters,FAB subtypes,karyotypes and other gene mutations in AML.The overall survival (OS) of AML patients with U2AF1 mutation (median 3 months) was shorter than those without mutation (median 7 months) (P=0.035).No difference in the OS was observed between MDS patients with and without U2AF1 mutations.Conelusion Our data show that U2AF1 mutation is a recurrent event at a low frequency in AML and MDS.