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Di19(drought-induced protein19)family is a novel type of Cys2/His2 zinc-fimger proteins.In this study,phosphorylation assay demonstrated that cotton Di19-1 and Di19-2(GhDi19-1/-2)proteins could be phosphorylated in vitro by the calciumdependent protein kinase(CDPK)in a Ca2+-dependent manner.Mutation of serine(Ser)site in N-terminus of GhDi 19-1 and GhDi 19-2 led to the altered subcellular localization of the two proteins in cells.GhDi19-1 and GhDi19-2 overexpression transgenic Arabidopsis seedlings displayed the hypersensitivity to high salinity and abscisic acid(ABA).