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The objective of this study was to investigate the correlation between cattle breed and deposit of adipose tissues in different position and the gene expression of fatty acid synthase (FASN), which was associated with lipid metablism and is valuable for understanding the physiology in fat depot and meat quality.Yanbian yellow cattle and Yah yellow cattle reared under the same conditions display different fat proportions in the carcass.To understand this difference, the expression of FASN in different adipose tissues and longissimus dorsi muscle (LD) in these two breeds were analyzed using Real-time quantitative polymerase chain reaction method(qRT-PCR).The result showed that FASN mRNA expression levels in subcutaneous fat and abdomianl fat in Yan yellow cattle were significantly higher than that in Yanbian yellow cattle.Furthermore, the expressions of FASN gene in lung, colon, kidney, liver, heart of Yanbian yellow cattle and Yan yellow cattle were also investigated.The results showed that the highest expression levels of FASN gene were detected in the lung in both breeds.