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  5. Quantitative mitochondrial proteomics by isobaric tags for relative and absolute quantification (iTR

Quantitative mitochondrial proteomics by isobaric tags for relative and absolute quantification (iTR

来源 :2013国际猪繁殖与呼吸综合征学术研讨会 | 被引量 : 0次 | 上传用户：jm8888jm8888
【摘 要】
：
Porcine reproductive and respiratory syndrome virus (PRRSV) is the cause of an economically important swine disease that has been devastating the swine industry since the late 1980s.The swelling of mi
【作 者】
：
R.Luo L.R.Fang D.Wang S.B.Xiao 
【机 构】
：
Division of Animal Infectious Diseases, State Key Laboratory of Agricultural Microbiology,College of
【出 处】
：
2013国际猪繁殖与呼吸综合征学术研讨会
【发表日期】
：
2013年12期
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论文部分内容阅读
Porcine reproductive and respiratory syndrome virus (PRRSV) is the cause of an economically important swine disease that has been devastating the swine industry since the late 1980s.The swelling of mitochondrion in PRRSV-infected pulmonary alveolar macrophages (PAMs) was observed by electron microscope.To discover mitochondrial protein responses in PRRSVinfected PAMs,mitochondrial fractions were isolated and labeled with isobaric tags for relative and absolute quantification (iTRAQ) and subsequently liquid chromatography-tandem mass spectrometry (LC-MS/MS) was employed to quantitatively identify the differentially expressed proteins between the PRRSV-infected cells and the controls.A total of 176 cellular proteins isolated from mitochondrion that were significantly altered post-intection were identitied.
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