Nonstructural proteins 1beta and 11 play an important role in differential TNF-alpha production indu

• 作 者: Q.He Y.Li L.Wang X.N.Ge L.Zhou X.Guo H.C.Yang
• 机 构: Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, College of Veterinary
• 出 处: 2013国际猪繁殖与呼吸综合征学术研讨会
• 发表日期: 2013年12期

An unparalleled large-scale,atypical clinical outbreak of PRRS in China in 2006 was caused by a highly pathogenic PRRSV (HP-PRRSV).TNF-alpha has been shown to have an antiviral effect by inducing apoptosis in virus-infected cells and promoting inflammatory response.Previous studies have shown that different PRRSV stains exhibited distinct ability in TNF-alpha induction.In this study,we first confirmed differential TNF-alpha expression in pulmonary alveolar macrophages (PAMs) induced by different pathogenic PRRSV strains and then indentified the viral proteins involved in such effect,and furthermore,verified the role of such viral proteins using reverse genetic technologv to construct chimeric viruses bv exchanging the determinant viral proteins-coding region.