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Connectome is a comprehensive map of neuronal connections in the brain.Diffusion MRI is the only imaging tool that can acquire such information from living human brain noninvasively.Diffusion MRI measures water molecular diffusion at the microscopic scale.The signal of diffusion MRI carries information about the microscopic structures, one of which is the orientation of the axonal fibers.Knowing axonal fiber orientation at each pixel location, one can apply a tractography technique to reconstruct fiber tracts.The fiber tracts reconstructed by tractography have been validated to correspond to the axonal fiber tracts revealed by invasive trace injection study.