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Digitization is an emerging trend in biochemical analysis.The word "digitization" here does not mean computerization of measurements or data analysis.Instead, it refers to the digitization of quantitation assays.The number of molecules in analyte is discrete by its nature.Therefore, upon better detection sensitivity approaching the single molecule level, the concentration and number of analyte molecules show intrinsic discreteness.An emerging analytical method that makes use of this intrinsic discreteness is digital counting.