【摘 要】
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Objective To explore the effects of intravenous injection of bone marrow stem cells (BMSCs) and intraperitoneal injection granulocyte colony stimulating factor (G-CSF) to mobilize stem cells on treatm
【机 构】
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Brain Aging and Cognitive Neuroscience Laboratory of Hebei Province, Shijiazhuang 050031, China
【出 处】
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中国神经科学学会第九届全国学术会议暨第五届会员代表大会
论文部分内容阅读
Objective To explore the effects of intravenous injection of bone marrow stem cells (BMSCs) and intraperitoneal injection granulocyte colony stimulating factor (G-CSF) to mobilize stem cells on treatment of cerebral artery occlusion (MCAO) model in rats.Methods Modified Zea-longa suture method was applied on the SD rats to block the middle cerebral artery, thus establish the model of cerebral infarction.BMSCs were injected via the tail vein and G-CSF was injected intraperitoneal 24 h after MCAO.7 and 14 days after the cell transplantation and stem cell mobilization, the neurological motor and conginitive function was evaluated by Morris water maze test.The infarct volume, and the BrdU positive cell survival number, migration and distribution were detected by Paraffin a slice of the organization of the immunochemical staining.The therapeutic effect of MCAO rats was conducted in the two group rats.Results (1) The swimming speed of both BMSCs group and G-CSF group at the 7th day and the 14th day after transplantation were higher than the PBS group, while the time for searching platform were shorter, and the performance of G-CSF group was better than the BMSCs group.(2) Compared with the model group, the infarction size of BMSCs group and G-CSF group decreased at the 7th day and the 14th day, and the size of G-CSF group was smaller than BMSC group.(3) The number ofBrdU-positive cells of G-CSF group was more than BMSCs group at the 7th day and the 14th after the injection, and mobilized stem cells could be seen surrounding cerebral ischemia and at the corpus callosum.Conclusion Intraperitoneal injection of G-CSF and tail vein injection of BMSCs could improve the motor and cognitive function, decrease the infarction size, and promote BMSCs to migrate to the surrounding cerebral ischemia and corpus callosum.All the results suggest that the method of G-CSF by mobilized BMSCs may be better than transplantation BMSCs via vein injection for treatment of cerebral ischemia.
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