In vitro proliferation and chondrogenesis of rabbit bone marrow mesenchymal stem cells induced by BM

• 作 者: ChenJialei;XiangZhou;HuangFuguo;LiuMing;DuanXin;
• 出 处: 2015成都国际骨科论坛暨四川省医学会第19次骨科学术会议
• 发表日期: 2015年期

　　Purpose: Aim to prepare and evaluate the bone morphogenesis protein-7 (BMP-7) loaded controlled-releasing microspheres made by poly(lactide-co-glycolide) (PLGA).In addition, the proliferation and chondrogenesis of rabbit bone marrow mesenchymal stem cells(MSCs) induced by BMP-7-loaded PLGA microspheres as an novel alternative for cartilage tissue engeering would be researched in vitro.Mathods: Double emulsion method was applied in preparation of microspheres.The proliferation potential of third passage MSCs was detected by MTT assay and growth curve was gained based on the data from MTT assay.Compare to proliferation and chondrogenesis of MSC between BMP-7-loaded PLGA microspheres group and controlled group.