毕赤酵母过量表达重组蛋白发酵条件优化及其酶学性质研究

来源 :2015年中国微生物学会学术年会 | 被引量 : 0次 | 上传用户：x1010

【摘要】

：

脂肪酶是一类重要的生物催化剂,可以催化水解、酯交换、酯合成等反应,广泛应用于光学纯的手性药物中间体合成行业,毕赤酵母的高密度发酵过量表达重组蛋白,进行进一步生物转化具有重要意义.应用DNA重组技术将lipase A突变体AA基因克隆到毕赤酵母组成型表达载体pGAPZαA中,利用电击转化的方法将重组载体整合到毕赤酵母基因组上,获得阳性重组毕赤酵母.对重组毕赤酵母的发酵条件优化,经优化后确定其最佳表达

【作者】

：

王鸿江刘立明

【机构】

：

江南大学食品科学与技术国家重点实验室,无锡214122

【出处】

：

2015年中国微生物学会学术年会

【发表日期】

：

2015年5期

下载到本地 , 更方便阅读

下载此文赞助VIP

声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架

论文部分内容阅读

其他文献

Stereospecificity of Isolated Enoylreductase Domains from Modular Polyketide Synthase

In modules of polyketide synthases (PKSs) that recruit methylmalonate extender units,enoylreductase (ER) domains set the C-2 methyl branch to either S or R configuration.To scrutinize the stereospecif

会议

Isolation and Characterization of A Nicotine-degradation Bacterium SCUEC2 Strain

A nicotine-degrading bacterium,strain SCUEC2,was isolated from the tobacco field soil,using nicotine as the only carbon source,and it was identified as Pseudomonas monteilii.When the nicotine concentr

会议

The antiviral protein viperin restricts Japanese Encephalitis Virus via interaction with non-structu

Japanese encephalitis virus (JEV) is an arbovirus belonging to the family Flaviviridae,which can cause acute viral encephalitis in both humans and animals.Viperin (also known as cig5 and RSAD2),an ant

会议

草酸青霉ABC转运蛋白突变株库的构建及影响纤维素酶表达效应因子的筛选

纤维素是自然界最为丰富的可再生生物质资源,因此,利用纤维素酶将生物质转化为可发酵的糖类能有效缓解因资源短缺所面临的能源问题.草酸青霉是一种高产纤维素酶的丝状真菌,与纤维素酶表达相关的效应因子的发掘和功能研究对系统解析纤维素酶表达调控机制具有重要作用.我们对草酸青霉基因组序列的生物信息学分析,发现存在45个编码腺苷三磷酸结合盒转运蛋白(ATP-binding cassette transporter

会议

Microbial community analysis of endophyte associated with infection by Root-knot Nematode in tomato

Here we investigated root microbiome of tomato in association with infection by root knot nematodes,to observe the bacterial response to the pathogens and the host plant.Community analysis of the root

会议

Expression,purification and characterization of a novel laccase from Streptomyces griseorubens JSD-1

Streptomvces griseorubens JSD-1 is a novel isolate that could grow efficiently upon lignin,and the ligninolytic genes active in this bio-transformation were expected to be crucial.To investigate the m

会议

Model and Simulation on Biofilm development of Pseudomonas aeruginosa based Two Dimension Cellular A

Pseudomonas aeruginosa is Gram-negative and paradigm organism for the study of biofilms.Attention is the study on the development of Eaemginosa biofilms.Mathematical Modeling is used to study the deve

会议

Genome Mining the Marine-derived Streptomyces sp.LZ35 strain for Novel Natural Products and Biosynth

The whole-genome sequencing projects have revealed that the microbial secondary metabolome is far richer than once thought,even in actinomycetes,which are responsible for the production of nearly 70 ％

会议

Novel Screening Method of Phage-displayed Peptide with Potential for Noncompetitive Detection of Hyd

In the field of rapid detection of small chemical contaminants,the current research is focused on developing affinity ligand assisted biodetection,biosensor or biochip methods.The bottleneck for devel

会议

Enzymatic synthesis of intermediate of rosuvastatin using lipase A in non-aqueous media

An efficient biocatalytic synthesis of intermediate of rosuvastatin (IR) has been developed using a green catalytic route,conducted under mild conditions without additional chiral reagents.Enzyme scre

会议

毕赤酵母过量表达重组蛋白发酵条件优化及其酶学性质研究

其他学术论文