【摘 要】
：
Objective Aquaporins (AQPs) belong to the water-specific channel protein family and have been reported to involve in cell and its organelle swelling.It has been reported to involve in multiple functio
【机 构】
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Neuroscience Research Institute, Peking University; Key Laboratory for Neuroscience (Peking Universi
【出 处】
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中国神经科学学会第九届全国学术会议暨第五届会员代表大会
论文部分内容阅读
Objective Aquaporins (AQPs) belong to the water-specific channel protein family and have been reported to involve in cell and its organelle swelling.It has been reported to involve in multiple functions in CNS.Our study is to explore the role of AQP1 in the specific nuclear response of astrocytes to Glu.Methods Expression and localization of AQP1 in atrocytes were detected through RT-PCR, Western Blot, Immunostaining and AQP1-EGFP transfection.Overexpression of AQP1 and AQP1s inhibitor treatment were used to study the role of AQP1 in Glu-induced nuclear swelling in astrocytes.Results We found that aquaporin-1 (AQP1) was uniquely expressed and located on the nuclear membrane of astrocytes to facilitate Glu-induced nuclear swelling.Over-expression of AQP1 in astrocytes could accelerate and enhance nuclear swelling and if inhibited would prevent swelling.The AQP1 localization and Glu-induced nuclear swelling are both specific to astrocytes, and could not be found in any other cells type.These unique features were also observed in human astrocytes.Conclusion Our study revealed that AQP1 mediated a specific nuclear response of astrocytes to Glu that might serve to facilitate communication of excitatory information between neurons and astrocytes through an as yet unknown mechanism.
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