【摘 要】
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FT gene is an important factor of integration into flowering;it plays an important role in the regulation of flowering time aspect.DNA and RNA were extracte
【机 构】
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SchoolofChemistryandLifeSciences,ChuxiongNormalUniversity
【出 处】
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第六届细胞结构与功能的信号基础研讨会
论文部分内容阅读
FT gene is an important factor of integration into flowering;it plays an important role in the regulation of flowering time aspect.DNA and RNA were extracted from the leaves and flowers from ornamental plants Paeoniarockii,followed by measuring the purity and quality of DNA and RNA,respectively.The total RNA was used as a template for reverse transcription to obtain cDNA first strand,and then for FT gene cloning.The results showed that the total RNA was easier to be extracted from flowersthan that in leaves ofPaeoniarockii.RT-PCR was performed andFT cDNA was cloned by using primers (F:5-ATGCCTAGAAATAGGGATCCACTA-3,R: 5-TTATCTTCTTCCGCCTGACC-3),a 1000-bp single band was clearly visualized,which was to be sequenced,and sequence alignment was to be performed.
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