Inhibition of glypican-3 expression via RNA interference influences the growth and invasive ability

• 作 者: JIANRUAN[1]FEIYELIU[1]XIAOHUACHEN[2]PENGZHAO[3]NINGSU[1]GUOZHUXIE[1]JIANPENGCHEN[1]DAYONGZHENG[1]RONGCHENGLUO[1]
• 机 构: Department of Oncology, Nanfang Hospital,Southern Medical University
• 出 处: 2012年全国肿瘤分子标志学术大会与国际肿瘤转化医学论坛暨第七届中国中青年肿瘤专家论坛
• 发表日期: 2012年期

　　Glypican-3 (GPC3), a membrane-bound heparan sulfate proteoglycan, is found to be overexpressed in hepato-cellular carcinoma (HCC).The purpose of the present study was to investigate the possible role of GPC3 in the development of HCC.In this study,RNA interference (RNAi) with a GPC3 small hairpin RNA (GPC3 shRNA) was used to identify the effects of GPC3 on the regulation of malignant behaviors of HCC.MHCC97-H, a highly metastatic human HCC cell line in which GPC3 mRNA and protein levels were detected as the highest among the 4 HCC cell lines assessed in this study, and was thus selected as a cell model for in vitro and in vivo experiments.The results showed that down-regulation of GPC3 can significantly inhibit the proliferative and invasive ability of MHCC97-H.Compared with the parental HCC cells, GPC3-silenced cells exhibited attenuated capacities in developing tumors in nude mice, while the growth of tumor xenografts derived from these cells dramatically regressed.In conclusion, our results suggest that GPC3 contributes to the proliferation and metastasis of HCC and that it may be a potential molecular target for HCC treatment.