上海教学医院嗜麦芽窄食单胞菌分子流行病学分析

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  论文目的:研究上海地区成人患者嗜麦芽窄食单胞菌感染的流行特征,从而为嗜麦芽窄食单胞菌的感控提供数据支持。论文方法:收集2014年1月至2014年9月上海交通大学医学院附属仁济医院成人患者感染的非重复嗜麦芽窄食单胞菌,采用多位点序列分型(MLST)方法和脉冲场凝胶电泳(PFGE)方法分析其遗传相关性;用K-B纸片法测定5种抗生素(左氧氟沙星,复方新诺明,舒普深即头孢哌酮/舒巴坦,特治星即哌拉西林/他唑巴坦,米诺环素)的敏感性;传统PCR方法检测四个主要毒力基因(Stmpr1,Stmpr2,smf-1,Smlt3773 locus),及其携带情况;采用半定量生物膜形成试验分析其生物膜形成;同时搜集病人的临床信息,更加系统全面地了解嗜麦芽窄食单胞菌的感染现状。
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