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A fingerprinting approach was developed by UPLC-PDA-MS/MS for quality control of processed Aconiti Radix.This fingerprinting approach was based on the processing methods recorded in Chinese Pharmacopoeia for the purpose of reducing the toxicity and ensuring the clinical therapeutic efficacy.Hierarchical cluster analysis and principal component analysis were performed to evaluate the fingerprinting approach.The results showed that the well processed,unqualified processed and the unprocessed Aconiti Radix could be clustered reasonably corresponding to their processing degrees.The loadings plot shows the main chemical markers having the most influence on the discrimination amongst the qualified and unqualified samples were benzoylmesaconine,benzoylaconine,benzoylhypaconine,mesaconitine,hypaconitine,Aconitine and 10-OH-mesaconitine.The UPLC-DAD-ESI/MS characteristic fingerprints were established according to the well processed and purchased samples (qualified).