【摘 要】
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Purpose To explore 17 killer cell immunoglobulin-like receptors (KIRs) gene and their ligands (human leukocyte antigen HLA-A and-B) polymorphisms in the Kazak individuals.Methods: 17 killer cell immun
【机 构】
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Medical Genetic Institute of Henan Province, People's Hospital of Henan Province, Zhengzhou
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Purpose To explore 17 killer cell immunoglobulin-like receptors (KIRs) gene and their ligands (human leukocyte antigen HLA-A and-B) polymorphisms in the Kazak individuals.Methods: 17 killer cell immunoglobulin-like receptors (KIR) genes and KIR ligands (human leukocyte antigen HLA-A and-B) were detected by using a polymerase chain reaction-sequence-specific primer (PCR-SSP).Methods Neighbor-joining phylogenetic tree between the studing population and its neighboring ethnic groups was constructed using the observed carrier frequencies of 13 KIR loci by SPSS 13.0.Result In the 110 unrelated healthy Kazak individuals living in Xinjiang Uygur Autonomous Region of China, we found the observed carrier frequencies of the 13 KIR genes ranged from 0.16 to 0.96; KIR2DL4, 3DL2, 3DL3 and 3DP1 were found to be present in every individual.And a total of 48 different KIR gene profiles were also identified.Conclusion the present study findings reveal the high polymorphism of KIRs in the Kazak population, demonstrate the KIR/HLA association in the study population, and enrich the KIR and HLA gene resources.The obtained KIR data will further the understanding of genetic relationships among populations in different geographic areas, and assist in answering questions regarding KIR/HLA relationships.
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