【摘 要】
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Objective: Self-renewal and differentiation of endogenous stem cells(SCs)are essential for adult tissue homoeostasis and intrinsic healing capacity.In this study,we hypothesize that penis contains a s
【机 构】
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Peking University First Hospital,Beijing 100034,China
【出 处】
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华夏医学论坛·泌尿生殖2015暨亚太性医学年会2015
论文部分内容阅读
Objective: Self-renewal and differentiation of endogenous stem cells(SCs)are essential for adult tissue homoeostasis and intrinsic healing capacity.In this study,we hypothesize that penis contains a small population of endogenous SCs,which might help rejuvenation of damaged erectile function.Methods: In this study,60 newborn male rats were intraperitoneally injected with 5-ethynyl-2-deoxyuridine(EdU;50 mg/kg)for the purpose of tracking endogenous SCs.Twelve weeks later,48 rats underwent bilateral cavernous nerves injury and were randomized into gavage feeding of solvent(vehicle group)or icariside Ⅱ(0.5,1.5,and 4.5 mg/kg/day,respectively).Twelve sham-operated rats received vehicle treatment and served as control.The treatments were continued for 4 weeks followed by a washout period of 72 h.Results: Results showed that ICA Ⅱ treatment significantly restored erectile function and effectively prevented distortion of normal neural anatomy,smooth muscle atrophy,and collagen deposition compared with the vehicle group.The numbers of label-retaining cells(LRCs)co expressing EdU and differentiated phenotypes(smooth muscle marker a-SMA or Schwann cell marker S100)were significantly higher in the three ICA Ⅱ-treated groups than those in vehicle group in a dose-dependent manner.In addition,the changing trend of p38 mitogen-activated protein kinase(MAPK)activity in the penis between groups was same as that of the number of differentiated LRCs.Conclusions: Together,these results suggest that the underlying mechanisms of ICA Ⅱ in ameliorating erectile function and pathological changes appear to involve enhanced endogenous SCs differentiation,which might be regulated by p3 8 MAPK signaling pathway.
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