【摘 要】
：
Aflatoxins are the most thoroughly studied mycotoxins produced by several members of the genus Aspergillus in section Flavi,Aspergillus flavus,Aspergillus p
【机 构】
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InstituteofLifeScience,NanchangUniversity,Nanchang330031,P.R.China;LehrstuhlfürTechnischeMikrobiolog
【出 处】
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2014年全国生物技术与食品质量博士后学术论坛
论文部分内容阅读
Aflatoxins are the most thoroughly studied mycotoxins produced by several members of the genus Aspergillus in section Flavi,Aspergillus flavus,Aspergillus parasiticus and Aspergillus nomius are aflatoxin producers frequently isolated from contaminated food sources.In order to identify and detect the three species and another non-aflatoxin producing species Aspergillus caelatus which is frequently isolated from Brazil nuts,we developed and evaluated a novel method,loop-mediated isothermal amplification(LAMP)assay,in separate analyses in which the target genes are acl1-genes of A.flavus and A.caelatus and amy1-genes of A.nomius and A.parasiticus.The detection limits for pure DNA of A.flavus,A.nomius,A.parasiticus and A.caelatus were 2.4,7.6,20 and 458 pg/reaction,respectively.
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