【摘 要】
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In apple,more than 80% of the total carbon flux goes through fructose in sink cell because sorbitol is a predominant translocated carbohydrate from source leaves.Compared with other plant species,appl
【机 构】
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State Key Laboratory of Crop Stress Biology in Arid Areas/College of Horticulture, Northwest A&F Uni
【出 处】
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第六届全国果树分子生物学学术研讨会
论文部分内容阅读
In apple,more than 80% of the total carbon flux goes through fructose in sink cell because sorbitol is a predominant translocated carbohydrate from source leaves.Compared with other plant species,apple may have stronger fructokinase (FK) activity to utilize fructose.To investigate the functions of FK isozyme gene in apple,we cloned four genes encoding FK,which were respectively homology gene of tomato LeFK1 to LeFK4.The analysis of mRNA expression and its promoter activity showed that MdFK2 was specifically expressed in shoot tips and young fruit,while its expression can be induced by anxin,fructose and glucose.Purified heterologous MdFK2 protein expressed in E.Coli showed high affinity to fructose (Km=0.08 mM),and no activity to glucose.MdFK2 activity could be significantly inhibited by fructose concentrations exceeding 2 mM,whereas max activity was detected at 1 mM fructose.In transgenic apple of MdFK2 with 35::SS promoter,MdFK2 expression increased FK activity,and all of Fructose,Glucose and Sucrose concentrations were significantly reduced in leaves of transgenic apple,but on influence on sorbitol concentration.Additionally,the transgenic apple showed high sensitive to damage from aphid and other stresses.
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