【摘 要】
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The sorbitol/sucrose (Sor/Suc) ratio in apple (Malus domestica) affects sugar homeostasis and metabolism.We used fruits from transgenic plants with reduced Sor synthesis in their leaves to investigate
【机 构】
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State Key Laboratory of Crop Stress Biology in Arid Areas/College of Horticulture, Northwest A&F Uni
【出 处】
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第六届全国果树分子生物学学术研讨会
论文部分内容阅读
The sorbitol/sucrose (Sor/Suc) ratio in apple (Malus domestica) affects sugar homeostasis and metabolism.We used fruits from transgenic plants with reduced Sor synthesis in their leaves to investigate sugar concentrations and the activities of enzymes related to sugar metabolism.We also analyzed the transcript abundance of various gene families that encode enzymes or transporters involved in sugar metabolism in fruits from MdA6PR antisense lines and an untransformed control.Because Sor is an alternatively transported carbohydrate,its decreased synthesis in the leaves did not obviously inhibit plant growth and development,and did not affect the total accumulation of dry matter in the fruit.The decreased ratio of Sor/Suc imported into the fruit largely improved the concentrations of glucose,whereas both Suc and fructose levels were only slightly changed.In addition,the reduced import of Sor diminished the activities of sorbitol dehydrogenase and fructokinase in the fruit.By contrast,enhanced movement of Suc up-regulated the activities of neutral invertase,sucrose synthase,and hexokinase.After an exhaustive analysis of sugar metabolism genes,we determined that the expression of MdSOT1,MdSDH,MdFK2,and MdSPS3 was down-regulated in fruits from transgenic plants.However,increased import of Suc markedly improved the expression of several genes of interest,including MdSUC1,MdSUC4,MdSUSY1,MdNIV3,MdHKs,and MdTMT1.These results provide new insight into the roles of Sor and the regulatory mechanism for sugar metabolism in apple fruit.Our findings also suggest possible functions for various gene families in controlling sugar homeostasis and the development of sweet flavors.
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