基于芯片技术的抗氧化剂对肿瘤微环境作用研究

来源 :2016全国生命分析化学学术大会 | 被引量 : 0次 | 上传用户：uuu_uuu

【摘要】

：

在本研究中,设计了一种具有三维动态共培养功能的微流控芯片用于肿瘤微环境模拟和体外抗氧化剂对恶性胶质瘤的功效研究[1]。芯片由五个平行且填充大孔水凝胶的通道组成。培养基和抗氧化剂溶液通过由软模法形成的管状腔灌注并渗透到水凝胶中[2]。在研究中,U87细胞和HUVEC细胞被成功应用于芯片上的三维动态共培养模拟,肿瘤细胞中谷胱甘肽和活性氧的变化作为判断细胞间作用和抗氧化剂作用效果的指标。

【作者】

：

刘汉阳介明沙何子怡张婉玲王俊茗李海芳林金明

【机构】

：

清华大学化学系,微量分析测试方法与仪器研制北京市重点实验室,北京,100084

【出处】

：

2016全国生命分析化学学术大会

【发表日期】

：

2016年4期

【关键词】

：

微流控芯片三维共培养抗氧化微环境水凝胶

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基于芯片技术的抗氧化剂对肿瘤微环境作用研究

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