CasOT: a genome-wide potential Cas9/gRNA off-target searching tool

来源 :第三届全国斑马鱼研究大会 | 被引量 : 0次 | 上传用户：a1263951733

【摘要】

：

CRISPR/Cas system,or Cas9/gRNA system,is a newly developed and highly effective tool for gene targeting used in many organisms and cultured cells.This system is very easy to design or engineer and it

【作者】

：

An Xiao Zhenchao Cheng Lei Kong Zuoyan Zhu Shuo Lin Ge Gao Bo Zhang

【机构】

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China Key Laboratory of Cell Proliferation and Differentiation of the Ministry of Education

【出处】

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第三届全国斑马鱼研究大会

【发表日期】

：

2013年12期

【关键词】

：

CRISPR-Cas Cas9-gRNA gene targeting engineered endonuclease

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　　CRISPR/Cas system,or Cas9/gRNA system,is a newly developed and highly effective tool for gene targeting used in many organisms and cultured cells.This system is very easy to design or engineer and it promotes the broad and fast-developing application of engineered endonuclease(EEN)technology in many model animals such as zebrafish.However,the specificity of Cas9/gRNA still needs careful evaluation.For example,it is reported to show considerable off-targeting effects in cultured human cells.

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CasOT: a genome-wide potential Cas9/gRNA off-target searching tool

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