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Gene trapping is a high-throughput approach to elucidate gene functions by disrupting and recapitulating expression of genes in a target genome.A number of transposon-based gene-trapping systems are developed for mutagenesis in cells and model organisms,but there is still much room for the improvement of their efficiency in gene disruption and mutation.Recently,a gene-trapping system mediated by Sleeping Beauty(SB)transposon was developed by inclusion of three functional cassettes.The mutation cassette can abrogate the splicing of trapped genes and terminate their translation.