Massively parallel quantification of miRNA in single cells via duplex-specific nuclease reaction in

来源 :2016年大连国际微纳流体和微流控芯片大会(2016 International Conference of Micro | 被引量 : 0次 | 上传用户：qinqinlian1982

【摘要】

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【作者】

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S.Subramanian Parimalam R.Yokokawa H.Kotera H.Shintaku

【机构】

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Department of Micro Engineering,Graduate School of Engineering, Kyoto University,C3-c2A06, Kyoto dai

【出处】

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2016年大连国际微纳流体和微流控芯片大会(2016 International Conference of Micro

【发表日期】

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2016年12期

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　　Novelty:We report a one step assay for the parallel quantification of miRNA in tens of thousands of single cells.Our protocol leverages DSN (duplex-specific nuclease) induced signal amplification (Fig.1) [1] and uses pico-liter sized micro-wells, which confine single cells and the enzymatic reaction.We demonstrated our assay with triplexed miRNA detection in K562 cells and a detection limit of miRNA at 5.7 copies per well.

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