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Somatic cell nucleus transfer (SCNT) has been considered the most effective method for conserving endangered animals and expanding the quantity of adult animal models.Bama miniature pigs are genetically stable and share similar biological features to humans.These pigs have been used to establish animal models for human diseases,and for many other applications.However,there is a pau-city of studies on the effect of ear fibroblasts derived from different age of adult Bama miniature pigs on nucleus transfer (NT).