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目的:观察珍菊降压片及其处方中西药组分对中枢神经系统、心血管系统、呼吸系统的影响,探寻中西药配伍增(存)效减毒的实验证据。方法:ICR小鼠随机分为珍菊降压片(ZJT)低、中、高剂量组(280,1 120,4 480 mg·kg~(-1)),另设相应剂量的珍菊降压片中药部分(ZJTZ)低、中、高剂量组(274,1 095,4 380 mg·kg~(-1)),珍菊降压片西药部分(ZJTX)低、中、高剂量组(6.2,24.9,99.5 mg·kg~(-1)),正常组,另设安定组。Beagle犬ZJT低、中、高剂量组(60,240,960 mg·kg~(-1)),另设相应剂量的ZJTZ低、中、高剂量组(58.7,235,939 mg·kg~(-1)),ZJTX低、中、高剂量组(1.3,5.3,21.3 mg·kg~(-1)),正常组。给ICR小鼠灌服,或给Beagle犬吞服珍菊降压片及其中药或西药部分,观察小鼠给药后自发活动状况以及协同戊巴比妥钠阈下催眠剂量对催眠的影响,记录和比较Beagle犬在给药前,给药后0.5,1,2,4,8,24 h的收缩压,舒张压,心率,PR间期,QRS波群,QT间期,T波形态,心律,呼吸频率和呼吸深度变化。结果:与正常组比较,ZJT灌胃给ICR小鼠280,1 120,4 480 mg·kg~(-1),受试动物自发活动下降,与戊巴比妥钠阈下催眠剂量有协同催眠作用,可缩短睡眠潜伏期,延长睡眠时间(P<0.05,P<0.01);吞服给Beagle犬960 mg·kg~(-1),受试动物收缩压、心率、呼吸频率和呼吸深度下降,其中血压下降起始时间为1 h,作用持续时间24 h。拆方研究结果显示,ZJT高剂量口服给药产生的收缩压、心率和呼吸频率和呼吸深度下降,以及显著的镇静作用,主要源于组方中的西药部分,给予ZJT,可显著增强降压活性,延长降压时间,阻断PR间期延长(P<0.05,P<0.01)。结论:ZJT高剂量口服给药,可使受试动物收缩压下降,其程度与剂量呈正相关,但同时对心率和呼吸有一定的抑制作用。此外,ZJT具有显著的镇静作用。ZJT的上述作用主要源于组方中的西药部分,加入中药部分,可增强降压活性,延长降压时间,抑制PR间期延长,表明以中西药配伍为特征的ZJT具有一定的增效减毒作用。
OBJECTIVE: To observe the effects of Zhenju Jiangya Jiangya Tablet and its prescriptions on the central nervous system, cardiovascular system and respiratory system, and to explore the experimental evidence that the compatibility of Chinese and western medicines is effective and attenuated. Methods: ICR mice were randomly divided into two groups: low dose, middle dose and high dose of ZJT (280,120,480 mg · kg -1) (ZJTZ) low, middle and high dose group (274,1 095,4 380mg · kg ~ (-1)), ZJTX low, middle and high dose group , 24.9,99.5 mg · kg -1). In the normal group, another stable group was established. The low, medium and high doses of ZJT (60,240,960 mg · kg -1) and the corresponding doses of ZJTZ (58.7,235,939 mg · kg -1) and ZJTX Low, medium and high dose groups (1.3,5.3,21.3 mg · kg -1), normal group. To ICR mice fed, or to Beagle dogs swallow Zhenju antihypertensive tablets and its traditional Chinese medicine or western medicine part of the mice after administration to observe the spontaneous activity and synergistic with pentobarbital sodium sublingual hypnosis dose on hypnosis, The systolic blood pressure, diastolic blood pressure, heart rate, PR interval, QRS complex, QT interval, T wave morphology, Heart rate, respiratory rate and respiratory depth changes. Results: Compared with the normal group, ZJT intragastrically administered to ICR mice 280,120,480 mg · kg -1, the spontaneous activity of the test animals decreased, and pentobarbital sodium subthreshold hypnosis dose hypnosis (P <0.05, P <0.01). The swallowed 960 mg · kg -1 of Beagle dogs, the systolic blood pressure, heart rate, respiration rate and respiration depth of test animals decreased, The onset time of blood pressure drop was 1 h and the duration of action was 24 h. Demolition side study showed that ZJT high dose oral administration of systolic blood pressure, heart rate and respiratory rate and respiratory depth decreased, and significant sedation, mainly from the part of the western medicine group, given ZJT, can significantly enhance the antihypertensive Activity, prolonging the time of hypotension and prolonging the PR interval (P <0.05, P <0.01). Conclusion: ZJT high dose oral administration can reduce the systolic blood pressure of the test animals, the degree of which is positively correlated with the dose, but at the same time it has certain inhibition on heart rate and respiration. In addition, ZJT has a significant sedative effect. The above effects of ZJT mainly originated from the western medicine part of the prescription group. Adding traditional Chinese medicine part can enhance the antihypertensive activity, prolong the antihypertensive time and prolong the PR interval, indicating that the ZJT characterized by the compatibility of traditional Chinese and western medicine has some synergistic effects Toxic effects.