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Respiratory syncytial virus (RSV) infection is leading cause of acute respiratory tract disease in <5 years old children,to further understand RSV molecular properties and clinical characterization due to RSV infection,we investigated RSV infection in hospitalized children under 5 years old with respiratory tract infection (RTI) in 2014 in the First peoples Hospital of Chenzhou,Hunan province,China.Nasopharyngeal aspirates (NPA) or nasal swab (NS) were screened by indirect fluorescence,complete or partial RSV G gene were amplified by RT-PCR and sequenced.