【摘 要】
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We established an efficient technology for regenerating stable rice lines in high in resistant starch (RS) using anthers of F1 plants from, landraces ,Zhaxi
【机 构】
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InstituteofFoodCrops,JiangsuAcademyofAgriculturalSciences,JiangsuHighQualityRiceR&DCenter,NanjingBra
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We established an efficient technology for regenerating stable rice lines in high in resistant starch (RS) using anthers of F1 plants from, landraces ,Zhaxima(Oryza sativa var.indica,)×Nanjing 46(Oryza sativa var.japonica) crosses.The anthers of the F1 plants were selected as explants to study the callus induction, differentiation and regeneration rates on different media using cold pretreatment and exogenous hormones.After obtaining the regeneration plants, we also investigated the plant height, stomatal perimeter, subspecies type based on 35 indel markers, and RS content in the regenerant seed.We found that in vitro doubled haploid technology provides new rice inbred lines in 2 years with a regeneration frequency of 4.55%.
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