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Objective This study evaluated the vascular endothelial growth factor165 (VEGF165) sustained-releasing ability and bioactive performance, mechanical properties, and cytotoxicity of the multi-walled carbon nanotube (MWCNT)-porcine small intestinal submucosa (PSIS) composite scaffold in vitro, detected the optimal concentration of MWCNT in the scaffold, and evaluated the feasibility of repairing abdominal wall defects using the composite scaffold.