Using CRISPR/Cas9 Repair HBB Gene Mutation In β-Thalassemia Ips Cells From β-Thalassemia Patient And

来源 :第十四次全国医学遗传学学术会议 | 被引量 : 0次 | 上传用户：kinganguo

【摘要】

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【作者】

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Niu Xiaohua Song Bing He Wenyin Ou Zhanhui Fan Yong Sun Xiaofang Sun Xiaofang

【机构】

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Key Laboratory for Major Obstetric Diseases of Guangdong Province, Key Laboratory of Reproduction an

【出处】

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第十四次全国医学遗传学学术会议

【发表日期】

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2015年9期

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　　Purpose : In this essay, we explore whether CRISPR/CAS9 could correct the HBB mutation of β-Thal iPSCs.Methods: In this study, firstly, we correct the disease-causing mutations in bThal iPSCs through in situ gene targeting using the CRISPR/Cas9 system.After gene targeting, 18 clones were picked and expanded.They were then verified by PCR for HygR fragment integration into the genomic DNA.

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Using CRISPR/Cas9 Repair HBB Gene Mutation In β-Thalassemia Ips Cells From β-Thalassemia Patient And

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