Cloning, expression of the CP2 gene of Taura syndrome virus and screen ligands from phage display ra

来源 :第十届全国病毒学学术研讨会 | 被引量 : 0次 | 上传用户：awaydedao132

【摘要】

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Objective: The major capsid protein CP2 of Taura syndrome virus (TSV) was cloned and expressed, peptides binding to CP2 were successfully obtained from a phage

【作者】

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HUANG Xin-xin Kong Fan-De CAI Qiang LU Cheng-ping

【机构】

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YangzteDeltaRegionInstituteofTsinghuaUniversityXiamenEntry-ExitInspectionandQuarantineBureauNanjingA

【出处】

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第十届全国病毒学学术研讨会

【发表日期】

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2013年9期

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Objective: The major capsid protein CP2 of Taura syndrome virus (TSV) was cloned and expressed, peptides binding to CP2 were successfully obtained from a phage display random dodecapeptide library.Methods: According to the nucleotide sequence of recombinant plasmid pMD-CP2, a pair of primer was designed to amplify the high variable region 376～1371bp of CP2 gene.The product was purified and cloned into the prokaryotic vector pET-32a to construct the recombinant plasmid,designated pET-CP2.

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Cloning, expression of the CP2 gene of Taura syndrome virus and screen ligands from phage display ra

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