Study of the annexin V-Nanoscale ultrasound contrast agents' preparation and targeting ultrasou

• 作 者: Tian ZHOU Ping ZHAO Wenbin CAI Hengli YANG Huizhong ZHANG Yunyou DUAN
• 机 构: Department of Ultrasound Diagnosis,Tangdu Hospital,Fourth Military University,Xi'an 710038,China
• 出 处: 第三届全国暨国际超声分子影像及生物效应和治疗学术会议
• 发表日期: 2016年9期

　　Objective To research the Annexin V-nanoscale ultrasound contrast agents preparation, and ultrasound imaging and the ability to bindg apoptosis cells of tumor in vitro.Methods The nanoscale bubble(Nanobubbles, NBs) packaged the Octaflouropropane(C3F8) gas was prepared by thin film hydration.The Annexin V-Nanobubbles (AVNBs) solutions was acquired through conjugating the biotinylated-Annexin V to the surface of the NBs by biotin-streptavidin bridging chemistry.The size and zeta potential of AVNBs were measured by NanoPlus-3 zeta/nano particle analyzer.The shift in size distribution of AVNBs bubbles was analyzed for the stability, when it was stored at 4℃ for different time.AVNBs shape was measured by scanning electron microscopy.The AVNBs bubble was measured using an ultrasound system for echogenicity in vitro, and SonoVue was for control.Finally, the ability of AVNBs binding with apoptosis cells of tumor in vitro was determined via the fluorescence microscope.Results AVNBs solutions were visible milky white and slightly transparent liquid with the naked eye.Under scanning electron microscopy,AVNBs were uniform hollow spherical cavitation bubble with small size and larger dispersability in solution.AVNBs has a size distribution of (640.2 ± 32.1)nm, and a mean zeta potential of (-23.30 ± 5.71) mV.Its size remained relatively constant and appeared to show less size variation within the 24h analysis period.The AVNBs and SonoVue solution had the same higher grayscale signal intensity by ultrasonic imaging.The AVNBs binded with apoptosis cells of tumor in vitro well, and the rate of binding was (97.55 ± 1.30)%.Conclusion The AVNBs particles prepared by method of thin film hydration have a nanoscale size, good stability and echogenicity.It can be targeted binding with the apoptosis cells of tumor in vitro.