Correlation between Autophagy and Apoptosis in Oral Carcinoma IL-24 Gene Therapy

• 作 者: LiJichen;
• 机 构: Department of Oral and Maxillofacial Surgery,the First Affiliated Hospital ofHarbin Medical Universi
• 出 处: 2014xi`an Conference of International Society for Maxillofac
• 发表日期: 2014年期

　　Purpose: To study the role of autophagy in the IL-24-induced apoptosis in oral carcinoma.Improve the effect of IL-24 gene therapy of oral carcinoma by inhibiting autophagy.Methods: In this study,apoptosis induced by IL-24 in human oral squamous cell carcinoma cell line(KB)was measured using the MTT assay,flow cytometry and Caspase-Glo(R)3/7 Assay.Autophagy activation induced by IL-24 was revealed Transmission Electron Microscope,monodansylcadaverine(MDC)staining,GFP-LC3 fluorescence and western blot assay of microtubule-associated protein 1 light chain 3(LC3).