Serum metabolomic profiling of piglets infected with virulent classical swine fever virus

• 作 者: Wenjie Gong Junjie Jia Bikai Zhang Shijiang Mi Li Zhang Xiaoming Xie Huancheng Guo Jishu Shi Changchun Tu
• 机 构: Institute of Military Veterinary Medicine, Academy of Military Medical Sciences, Changchun 130122, C
• 出 处: 2016第六届中国兽药大会(中国畜牧兽医学会动物药品学分会第五届全国会员代表大会暨2016学术年会、中国微生物学会兽医微
• 发表日期: 2016年12期

　　Classical swine fever (CSF) is a highly contagious swine infectious disease and causes significant economic losses for the pig industry worldwide.The objective of this study was to determine whether small molecule metabolites contribute to the pathogenesis of CSF..Birefly,serum metabolomics of CSFV Shimen strain-infected piglets were analysed by ultraperformance liquid chromatography/electrospray ionization time-of-flight mass spectrometry (UPLC/ESI-QTOF/MS) in combination with multivariate statistical analysis.In CSFV-infected piglets at days 3 and 7 post infection changes were found in metabolites associated with several key metabolic pathways, including tryptophan catabolism and the kynurenine pathway, phenylalanine metabolism, fatty acid and lipid metabolism, the tricarboxylic acid and urea cycles, branchedchain amino acid metabolism, and nucleotide metabolism.Several pathways involved in energy metabolism including fatty acid biosynthesis and β-oxidation, branched-chain amino acid metabolism and the tdcarboxylic acid cyle were significantly inhibited.Changes were also observed in several metabolites exclusively associated with gut microbiota.The metabolomic profiles indicate that CSFV-host gut microbiome interactions play a role in the development of CSF.