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The malonyl-CoA:acyl carrier protein transacylase (MCAT), a key enzyme in the FAS Ⅱ pathway, is responsible for transferring the malonyl group from malonyl-CoA to the-SH group of ACP and then forming the free CoASH and malonyl-ACP.Malonyl-ACP is the two-carbon donor required for the elongation steps in fatty acid biosynthesis.Previous studies indicate that the MCAT enzyme performs a two-step reaction in which MCAT first binds to malonyl-CoA to form a stable tetrahedral malonyl-MCAT intermediate; the holo-ACP then docks on the surface of the intermediate via a hydrophobic interaction to receive the malonyl chain.In fact, MCAT induces an upstream reaction and may be the essential building block in the fatty acid biosynthesis cycle.