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The derivation of female germ cells provides the capability to study human oogenesis.Previously, we reported that female germ cells were triggered from CD117+/CD44+ human amniotic fluid stem cells (hAFSCs), which were cultured in the conditioned medium, supplemented with 5% porcine follicle fluid (pFF) for 10 days.The monolayer cells were changed to form cell aggregates, which with the morphological characteristics of early female germ cells were loosened off into medium incessantly.Cell aggregates were collected and then replated on the fresh dish with oocyte growth medium for additional 7-10 days.The cumulus-oocyte-complexes (COCs) like structures from aggregates were derived and oocyte-like cells (OLCs) ranged from 50-120 μm with the zona pellucida were observed, some OLCs could develop spontaneously into multi-cell structures similar to preimplantation embryos, which expressed the folliculogenesis and oogenesis associated markers.Recently, the high level of bone morphogenetic protein (BMP15) was detected in pFF, which could be helpful to demonstrate how these hAFSCs differentiated to female germ cells.Meanwhile, these female germ cells-like cells were packaged into porcine theca folliculi to form graft, and then which was transplanted into mouse renal capsule.The results showed that the morphology of cells from the graft after transplantation was similar to normal oocytes, germ cells specific genes (OCT4, NANOG, STELLA,IFITM3, DAZL, NANOS3, BMP15, and GDF9) were detected positive, some OLCs from the graft were observed with expression of BMP 15 by immunofluorescence.Although the in vitro fertilization potentials are unproven yet, the derivation of OLCs from hAFSCs, as a result of short term (<25 days) and high efficiency (>2%), may provide a new ideal approach to study human female germ cell development in vitro.