转录因子ZEB1在结肠癌组织中的表达及其对结肠癌细胞HCT116增殖和侵袭能力的影响

来源 :中国免疫学会第九届全国免疫学学术大会 | 被引量 : 0次 | 上传用户：myrost

【摘要】

：

【作者】

：

郑国旭史圣甲魏铭席文锦郭张燕杨帆孟艳玲温伟红杨安钢

【机构】

：

第四军医大学基础部免疫学教研室第四军医大学西京医院泌尿外科

【出处】

：

中国免疫学会第九届全国免疫学学术大会

【发表日期】

：

2014年8期

【关键词】

：

E盒锌指结合蛋白1 ZEB1 结肠癌预后治疗靶点

下载到本地 , 更方便阅读

下载此文赞助VIP

声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架

论文部分内容阅读

研究背景:E盒锌指结合蛋白1 (Zinc finger E-box binding homeobox 1,ZEB1)在多种肿瘤中高表达,在某些肿瘤中可以作为预后判断的标志分子.然而,ZEB1在结肠癌中的临床意义尚不明确.目的:在结肠癌组织样本中检测ZEB1的表达水平,分析其与临床病理特征的相关性;在结肠癌HCT116细胞中下调ZEB1的表达,分析其对细胞生物学功能的影响.方法:用免疫组织化学的方法检测了108例结肠癌患者的石蜡包埋组织切片中ZEB1的表达,用生存分析及COX回归分析探讨其表达水平与结肠癌临床病理特征的相关性;用siRNA沉默ZEB1的表达,观察其表达下调对HCT116细胞增殖、凋亡、迁移和侵袭能力的影响.

其他文献

SIRT1 limits the function and fate of myeloid-derived suppressor cells in tumors by orchestrating HI

Myeloid-derived suppressor cells (MDSC) display an immature phenotype that may assume a classically activated (M1) or alternatively activated phenotype (M2) in tumors.In this study, we investigated me

会议

Targeted inhibiting REG3A/JAK2/STAT3 positive-feedback loop effectively blocks the progression of pa

Background : Pancreatic cancer is an aggressive disease with a high rate of cell metastasis.Regenerating islet-derived protein 3-alpha (REG3A), a secreted pancreas protein and cell proliferation facto

会议

Pancreatic cancerSTAT3REG3ACell growthFeedback loop

Pro-apoptotic and anti-proliferative effects of mitofusin-2 via PI3K/Akt signaling in breast cancer

Mitochondrial GTPase mitofusin-2 gene (Mfn2) is a novel gene characterized as a cell proliferation inhibitor.Recently, Mfn2 has been reported to have a potential role in regulating cell proliferation,

会议

Delivery of chemotherapeutic drug by tumour cell-derived microparticles reverses drug resistance of

Stem cell-like cancer cells, a minor population in tumour mass acting as the source of tumour recurrence and metastasis, are extraordinarily resistant to chemotherapeutic drugs, leading to a major cli

会议

胃癌中NK细胞功能失调的机制研究

背景:胃癌是全球排名第四的恶性肿瘤,免疫抑制微环境的建立与持续性存在是导致胃癌发生发展的重要因素,我们前期的研究发现胃癌中CD8+T细胞功能受到抑制并介导了胃癌的不断进展和预后不良,而作为另一重要的抗肿瘤免疫细胞即NK细胞在胃癌中的功能和调控并不清楚.目的:研究NK细胞在胃癌中的功能变化及其调控,进一步阐明胃癌免疫逃逸的机制.方法:流式细胞技术检测NK细胞在胃癌患者不同组织中的分布、表型及功能,流

会议

胃癌NK细胞进展

PD-L1 (CD274) protein expression in ovarian carcinoma: the correlation with degree of differentiatio

Background: In spite of its reputation as a T cell regulator, little is known about the PD-L 1 acting as a tumor marker.Method: Here, the relationship between cell differentiation and changes in the s

会议

PD-L1ovarian carcinomadifferentiation

Inhibitory effect of epirubicin-loaded lipid microbubbles bound with anti-ABCG2 antibody combined wi

To investigate the inhibitory effect of epirubicin (EPI)-loaded lipid microbubbles (MBs) bound with anti-ABCG2 monoclonal antibody (EPI-MBs+mAb) combined with therapeutic ultrasound on multiple myelom

会议

multiple myelomacancer stem cellsmicrobubbleepirubicinABCG2

Recombinant Trimeric H5N1-HA Vaccine Induces Protective Immunity that Primarily Targets the Receptor

Highly pathogenic avian influenza A virus H5N1 continues to spread among poultry and has frequently broken the species barrier to humans, calling for effective vaccines and therapeutics.In this work,

会议

H5N1 virusHANeutralizing antibodyVaccine

DNA vaccine encoding Hantavirusneucleoprotein (NP) with LAMP chimera, targeted to the MHC class Ⅱ pa

Background : Endogenous antigen encoded by conventional naked DNA was processed by antigen-presenting cells (APCs) and only presented to CD8+ T cell through forming peptide/MHC Ⅰ (major histocompatibi

会议

NPLAMPDNA vaccineELISpotCytotoxicity

MicroRNA-155与NK/T细胞淋巴瘤临床特征的关系及对人NK/T细胞淋巴瘤细胞株SNK-6的生长调控的研究

目的:通过检测miR-155在NK/T细胞淋巴瘤患者外周血及人NK/T细胞淋巴瘤细胞株SNK-6细胞中的表达,探索其与NK/T细胞淋巴瘤临床特征的相关性及其可能的作用机制,以期寻找NK/T细胞淋巴瘤新的肿瘤标记物及预后预测指标.方法:1.研究对象:患者血液标本来自于2013年～2014年3月间,就诊于河北医科大学第四医院血液科的NK/T细胞淋巴瘤患者.所有患者均签署知情同意书.2.人NK/T细胞淋

会议

NK/T细胞淋巴瘤microRNAmiR-155Total RNANF-κB (p65)

转录因子ZEB1在结肠癌组织中的表达及其对结肠癌细胞HCT116增殖和侵袭能力的影响

其他学术论文