【摘 要】
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The successful development of in vitro assays with cultured pulmonary cells and aerosols is instrumental for creating toxicity/screening tests for use during product development.To optimize for future
【机 构】
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DuPont Haskell Global Centers for Health & Environmental Sciences
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The successful development of in vitro assays with cultured pulmonary cells and aerosols is instrumental for creating toxicity/screening tests for use during product development.To optimize for future in vitro aerosol exposures using the Nano-Aerosol Chamber for In Vitro Toxicology (NACIV-T) system, rat lung epithelial L2 cells and NR8383 macrophages (M(O)) were co-cultured on an ALl system and cell metabolism (XTT), cytotoxicity (LDH), and cytokine (IL-6) release assays were developed as endpoints to assess cell toxic effects following particle exposures.Co-cultures were exposed to 0-80 μg· cm-2 ZnO fine particles in supplemented growth medium for 2 h followed by 24-or 48-h maintenance of cultures at ALI.
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