Synthesis and Application of a Novel Fluorescent pH Probe 4,4'-bis(3-aminophenyl)-2,2'-bit

来源 :中国化学会第十三届全国有机合成化学学术研讨会 | 被引量 : 0次 | 上传用户:abwyn12
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  A novel ratiometric fluorescent pH probe 4,4-bis(3-aminophenyl)-2,2-bithiazole (BABT) was synthesized and its structure was certified by 1H NMR,13C NMR and LCMS.As shown in Fig.1,the pH titration indicates remarkable fluorescence enhancement effect with pH decreasing from 7.40 to 1.45 and therefore BABT is a useful fluorescent probe for strongly acidic pH sensing.This probe also exhibits high selectivity,excellent stability and reversible response according to Fig.2,Fig.3 and Fig.4,respectively.Furthermore,laser scanning confocal microscopy of Escherichia coli cells indicates that the probe can also be used for monitoring pH changes of the biological systems,as shown in Fig.5.
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