Applicability of Cloud Point Extraction Coupled with Back-extraction to the Determination of Organop

• 作 者: LuZhou;
• 机 构: College of Food Science and Engineering, Shandong Agriculture University, Tai'an271018, Shandong Pr
• 出 处: 第2届大连国际色谱学术报告会、第37届国际高效液相色谱及相关技术会议、第18届全国色谱学术报告会
• 发表日期: 2011年期

　　A procedure for the determination of organophosphorous pesticides (OPPs) in cider was developed using the cloud point extraction of nonionic surfactant (PEG 4000) coupled with back-extraction prior to Gas Chromatography-Mass Spectrometer (GC-MS) analysis.The parameters and variables that affect the extraction were investigated.Under optimum conditions: a solution containing 300 gL-1 polyethylene glycol 4000 (PEG 4000) and Na2SO4 for the extraction of the OPPs, the coacervation phase was obtained by back extraction with hexane.The upper hexane solution was vortex mixed simply for further cleanup for automatic injection.A preconcentration factor of 40 was obtained for these 45 pesticides.Using this method, we found that carbofuran residues could be determined with recoveries ranging from 71.2% to 116.9%, relative standard deviations (RSD) in the range of 1.2% to 14.7% for three levels, and the limits of quantification (LOQ) together with the limits the detection (LOD) were in the range of 0.02 to 3.00 and 0.01 to 0.91 μgkg-1 in cider, respectively.