【摘 要】
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Background To study the expressions of endothelin-1 at early stages of atrial fibrillation in a rapid paced primary cultured atrial myocyte model.Methods Primary rat atrial myocytes were cultured and
【机 构】
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Department of Congenital Heart Disease,Cardiovascular Research Institute of People's Liberation Arm
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Background To study the expressions of endothelin-1 at early stages of atrial fibrillation in a rapid paced primary cultured atrial myocyte model.Methods Primary rat atrial myocytes were cultured and a rapid paced cell model was established.The polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA) were applied to detect the messenger ribonucleic acid (mRNA) expression and secretion ofendothelin-1.
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