【摘 要】
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Protein denaturation and oligomerization have attracted significant interests because of its pivotal role in human diseases and drug safety.To understand the molecular mechanisms of these processes,va
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Protein denaturation and oligomerization have attracted significant interests because of its pivotal role in human diseases and drug safety.To understand the molecular mechanisms of these processes,various non-native protein conformers and oligomers involved at different stages should be characterized in detail.However,the heterogeneity and transient nature displayed by the co-existing species pose much challenge.Here we developed hybrid approaches to allow characterization of such systems at multiple levels.These approaches include determination of protein-binding stoichiometries and complex abundances using native mass spectrometry(MS),evaluation of protein thermostability using temperature-controlled MS,conformer-specific characterization using hydrogen/deuterium exchange(HDX)MS,and disulfide mapping using top-down MS.
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