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To observe the efffects of autophagy on expression of TNF-α and TGF-β in silica induced alveolar macrophages (AMs) injury in vitro.AMs collected from bronchoalveolar lavage fluid of SD rats were randomly divided into three groups:control group, model group and 3-MA group.AMs were exposed slica to establish cell model in vitro.AMs were incubated with 3-MA on the 1 st day of slica treatment.