Molecular cloning and response to bacteria infection of arginine kinase in the mud crab,Scylla param

• 作 者: Yinjie Xie haihui Ye huiyang Huang ya'nan Yang jingru Huang
• 机 构: College of Oceanography and Environmental Science, Xiamen University, Xiamen 361005, Fujian, PR Chin
• 出 处: 2011年中国水产学会学术年会
• 发表日期: 2011年4期
• 关键词: mud crab Arginine kinase Innate immunity Quantitative real-time PCR

　　Arginine kinase (AK) was previously reported as a phosphagen-ATP phosphotransferase found in invertebrate.In this study, an 1469bp cDNA was cloned and sequenced from Scylla paramamosain (designated SpAK).It contained an open reading frame of 1071bp that coded for 357 deduced amino acids of AK.The calculated molecular mass of SpAK is 40243.8 Da and PI is 5.92.