Apoptotic and non-apoptotic function of Caspase 7 during spermatogenesis

• 作 者: BinLei;XumingZhou;DaojunLv;ShouboZhang;FangpengShu;YanqingDing;YuanyuanZhang;XiangmingMao;
• 机 构: Department of Urology, Peking University Shenzhen Hospital, Shenzhen,Guangdong Province, China
• 出 处: CUA2015全国泌尿男生殖系统肿瘤专题会议(10th)暨2015年广东省医学会泌尿外科学学术年会
• 发表日期: 2015年期

　　OBJECTIVE Recent studies have reported that caspase 7 have apoptotic and non-apoptotic function.However, the relationship between caspase 7 and spermatogenesis remains unknown.This study aimed to investigate the possible function of caspase 7 during normal and abnormal spermatogenesis.METHODS The cleaved form of caspase 7 was detected in testis tissues at different postpartum times (5-14 weeks) by qRT-PCR,Westem blot and immunohistochemistry (IHC).Then, the mice models of spermatogenic dysfunction were obtained by busulfan (30mg/kg) to further evaluate the potential function and mechanism of caspase 7.RESULTS QRT-PCR and Western blot results showed that caspase 7 expression was gradually elevated from 5 to 14 weeks,which was not connected with apoptosis.