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MicroRNA-25 (miR-25) has been reported to be a major miRNA marker in neural cells and is strongly expressed in ischemic brain tissues.However,the precise mechanism and effects of miR-25 in cerebral ischemia/reperfusion injury needs further investigations.In the present study,oxygen-glucose deprivation models were constructed in human SH-SY5Y and IMR-32 cells to mimic cerebral ischemia/reperfusion injury and to evaluate the role of miR-25 in regulating oxygen-glucose depriva-tion/reperfusion-induced cell apoptosis.We found that miR-25 was down-regulated in oxygen-glucose deprivation/reperfusion models.Overexpression of miR-25 via miRNA-mimics transfection remarkably inhibited oxygen-glucose deprivation/reperfu-sion-induced cell apoptosis.Moreover,Caspase-3 and Bax protein expression were down-regulated by miR-25 overexpression in in vitro oxygen-glucose deprivation/reperfusion models with up-regulated Bcl-2 expression.Taken together,our results indicate that up-regulation of miR-25 inhibits cerebral ischemia/reperfusion injury-induced cell apoptosis through down-regulation of Caspase-3 and Bax,and up-regulation of Bcl-2,which will provide a promising therapeutic target for the treatment of cere-bral ischemia/reperfusion injury.