Pestalotiopsis fici genome reveals the richness of secondary metabolites of endophytic fungus

• 作 者: Xiuna Wang Xingzhong Liu Ling Liu Xinyu Zhang Gang Liu Liangdong Guo Yongsheng Che Chengshu Wang Zhiqiang An Liyun Guo
• 机 构: State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences,No 3 1st We
• 出 处: 2012年中国菌物学会学术年会
• 发表日期: 2012年8期
• 关键词: Endophytic fungi secondary metabolite genome

　　Endophytic fungi grow within their plant hosts without causing apparent disease symptoms and are an important source of novel bioactive secondary metabolites (Schulz, B.and C.Boyle 2006, Strobel, G., B.Daisy, et al.2004).Pestalotiopsisfici, an important woody plant endophyte, produces numerous bioactive secondary metabolites(Liu, L 2011).To reveal the mystery of so many secondary metabolites, the genome of P.fici was shotgun sequenced using a Roche 454 GS FLX system.There is a 51.91 Mb genome size containing ~ 16,063 predicted protein-encoding genes.Annotation indicated that the gene clusters for secondary metabolites were significantly expanded in number and diversity comparing with other fungal genomes.The protein families related to secondary metabolism, such as MFS general substrate transporter,aromatic compound dioxygenase, cytochrome P450, FAD-binding domain oxidase, ankyrin repeat,Zn2/Cys6 DNA-binding domain etc.are more abundant in the genome ofP.fici based on the Pfam and CAFE analysis.The preliminary analysis also found evidence for horizontal transfer of a secondary metabolite gene cluster from host plants to P.fici.This research explained that the richness of seccndary metabolites in endophytic fungi from gene expansion and horizontal transfer of gene clusters.