APG1387, a novel IAPs inhibitor, effectively inhibits properties of nasopharyngeal carcinoma cancer

• 作 者: 戢娇;余彦;邓蓉;陈文丹;张相强;焦琳;李轩;伍瑞燕;李宝霞;冯公侃;杨大俊;朱孝峰;
• 机 构: 中山大学肿瘤防治中心,华南肿瘤重点实验室,广东广州,510060
• 出 处: 2015医学前沿论坛暨第十四届全国肿瘤药理与化疗学术会议
• 发表日期: 2015年期

　　Purpose: To demonstrate the effects of a novel Smac/DIABLO mimetic APG1387 on properties of nasopharyngeal carcinoma cancer stem-like cells, and invest further to establish the mechanisms thereof.Method: FACS was used to sort SP cells.Suspension culture assay was performed to detect nasosphere.Western-blotting was used to detect the protein levels.Immunoprecipitation was employed to determine the protein-protein interactions.Molecular cloning and lipofectamine transfection technology was used for plasmid construction, transfection and RNA Interference.Results: We found that the S18 cell line had higher SP population portion and stronger ability of sphere-formation and metastasis, which suggests higher CSC-like properties than other NPC cell lines.